Hemileia vastatrix Pseudomonas syringae pv. Thus, our experimental approach is an effective tool for the characterization of effector/avirulence proteins of this important pathogen. Our findings suggest that HvEC-016 may be recognized by the plant immune system in a S H 1-dependent manner. By contrast, HvEC-016 enhanced bacterial multiplication in S H 1-lacking plants. Suppression of bacterial blight symptoms in S H 1 plants was associated with reduced bacterial multiplication. Using this Psgc-adapted effector detector vector (EDV) system, we found that HvEC-016 suppresses the growth of Psgc on coffee genotypes with the S H 1 resistance gene.
syringae T3SS secretion signal which enables us to translocate HvECs into the cytoplasm of coffee cells. Employing a calmodulin-dependent adenylate cyclase assay, we demonstrate that Psgc recognizes a heterologous P. vastatrix effector candidate genes (HvECs) expressed at different stages of its lifecycle, we established an assay to characterize HvEC proteins by delivering them into coffee cells via the type-three secretion system (T3SS) of Pseudomonas syringae pv. A number of genes that confer resistance to coffee leaf rust (S H 1-S H 9) have been identified within the genus Coffea, but despite many years of research on this pathosystem, the complementary avirulence genes of Hemileia vastatrix have not been reported.
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